Histopathological evaluation of hepatic tissue of yellow Rasbora (Rasbora lateristriata) exposed to paracetamol

Authors

  • Nur Indah Septriani Universitas Gadjah Mada
  • Ruth Liananda Citra Dolok Saribu Universitas Gadjah Mada
  • Tia Apriliyani Universitas Gadjah Mada
  • Ina Karlina Universitas Gadjah Mada
  • Nur Ainun Oktavia Pusparini Universitas Gadjah Mada
  • Laili Mufli Zusrina Universitas Gadjah Mada
  • Ria Vinola Septhya Sari Universitas Gadjah Mada
  • Hayu Swari Allimi Universitas Gadjah Mada
  • Murtiadi Erlan Supraitno Universitas Gadjah Mada
  • Faisal Saeed Universitas Gadjah Mada
  • Eben Ezer Simanungkalit Universitas Gadjah Mada
  • Pradnya Paramita Universitas Gadjah Mada
  • Bambang Retnoaji Universitas Gadjah Mada
  • Fajar Sofyantoro Universitas Gadjah Mada
  • Nastiti Wijayanti Universitas Gadjah Mada

DOI:

https://doi.org/10.31763/bioenvipo.v3i1.595

Keywords:

Hepar, Necrotic, Paracetamol, Picnotic, Yellow rasbora

Abstract

Excessive consumption of paracetamol may lead to hepatic necrosis, nephrotoxicity, extra-hepatic lesions, and even fatality in both humans and animals. The objective of this study was to examine the histological changes in the hepatocytes of yellow rasbora (Rasbora lateristriata) exposed to paracetamol. The fish were subjected to varying concentrations of paracetamol over a period of four days. Group I served as the control group without any paracetamol exposure. Groups II, III, and IV were exposed to 2, 3, and 4 mg/L of paracetamol, respectively. A total of six individuals were allocated to each of the four experimental groups. The histological analysis of the hepatic tissue following paracetamol exposure at concentrations of 2, 3, and 4 mg/L revealed hepatic damage characterized by picnosis, necrosis, and vacuolization. In summary, higher concentrations of paracetamol were associated with an increase in the severity of histopathological changes in the hepatic tissues of yellow rasbora.

Author Biographies

Nur Indah Septriani, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Ruth Liananda Citra Dolok Saribu, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Tia Apriliyani, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Ina Karlina, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Nur Ainun Oktavia Pusparini, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Laili Mufli Zusrina, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Ria Vinola Septhya Sari, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Hayu Swari Allimi, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Murtiadi Erlan Supraitno, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Faisal Saeed, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology
Department of Zoology, Faculty of Natural Sciences, Islamia College Peshawar

Eben Ezer Simanungkalit, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Pradnya Paramita, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Bambang Retnoaji, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Fajar Sofyantoro, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

Nastiti Wijayanti, Universitas Gadjah Mada

Department of Tropical Biology, Faculty of Biology

References

Rotundo, L. & Pyrsopoulos, N. Liver injury induced by paracetamol and challenges associated with intentional and unintentional use. World Journal of Hepatology 12, 125–136 (2020).

Islam, M. T. et al. Effects of nerol on paracetamol-induced liver damage in Wistar albino rats. Biomedicine & Pharmacotherapy 140, 111732 (2021).

Mazaleuskaya, L. L. et al. PharmGKB summary: pathways of acetaminophen metabolism at the therapeutic versus toxic doses. Pharmacogenetics and Genomics 25, 416–426 (2015).

Hodgman, M. J. & Garrard, A. R. A Review of Acetaminophen Poisoning. Critical Care Clinics 28, 499–516 (2012).

Bessems, J. G. M. & Vermeulen, N. P. E. Paracetamol (Acetaminophen)-Induced Toxicity: Molecular and Biochemical Mechanisms, Analogues and Protective Approaches. Critical Reviews in Toxicology 31, 55–138 (2001).

Adil, M. et al. Ameliorative effect of naringin in acetaminophen-induced hepatic and renal toxicity in laboratory rats: role of FXR and KIM-1. Renal Failure 38, 1007–1020 (2016).

Brune, K., Renner, B. & Tiegs, G. Acetaminophen/paracetamol: A history of errors, failures and false decisions: 130 years of aniline analgesics. European Journal of Pain 19, 953–965 (2015).

Guiloski, I. C. et al. Paracetamol causes endocrine disruption and hepatotoxicity in male fish Rhamdia quelen after subchronic exposure. Environmental Toxicology and Pharmacology 53, 111–120 (2017).

Djumanto, D. & Setyawan, F. Food habits of the yellow rasbora, Rasbora lateristriata,(family: Cyprinidae) broodfish during moving to spawning ground. Jurnal Perikanan Universitas Gadjah Mada 11, 107–116 (2009).

Sentosa, A. A. & Djumanto, D. Spawning habitat of Rasbora lateristriata in Ngrancah river, Kulon Progo regency. Jurnal Iktiologi Indonesia 10, 55–63 (2010).

Lailiati, I. R., Suci, D. A., Rosa, A. A., Fernanda, V. A. & Retnoaji, B. Reproductive Aspect and Embryonic Development of Wader Fish ( Rasbora lateristriata Bleeker, 1854) from Purworejo, Central Java: in Proceedings of the 7th International Conference on Biological Science (ICBS 2021) 540–544 (Atlantis Press., 2022).

Djumanto, D., Setyobudi, E., Sentosa, A. A. & Nirwati, N. Reproductive biology of the yellow rasbora (Rasbora Lateristriata) inhabitat of the Ngrancah river, Kulon Progo regency. Jurnal Perikanan Universitas Gadjah Mada, 10, 261–275 (2008).

Harris, A., Liliyanti, M. A., Sumahiradewi, L. G. & Artiningrum, N. T. Morphometric, Meristic Characters, And Gonad Mature Level of Pepudah Fish (Rasbora Lateristriata) from Sokong Rivers, North Lombok District. PENBIOS: Jurnal Pendidikan Biologi dan Sains 6, 28–37 (2021).

Wilkinson, C. L. & Hui, T. H. Fishes of the Brantian drainage, Sabah, Malaysia, with description of a new Rasbora species (Teleostei: Cyprinidae). Raffles Bulletin of Zoology 66, 595–609 (2018).

Anshori, K. & Retnoaji, B. Pengaruh Bahan Aktif Insektisida Chlorpyrifos terhadap Struktur Histologis Hepar Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854) (Effect of Active Insecticide Chlorpyrifos on the Histological Structure of the Liver of Wader Fish (Rasbora lateristriata Bleeker, 1854)). Berita Biologi 21, 51–59 (2022).

Raharjeng, A. & Retnoaji, B. The Effect of Dioscorea alata Extract on the Early Development of Zebrafish Embryo (Danio rerio) and Rasbora lateristriata: in First International Conference on Science, Technology, Engineering and Industrial Revolution (ICSTEIR 2020) (2021). doi:10.2991/assehr.k.210312.096.

Retnoaji, B. et al. The effect of volcanic dust on the histological structure of wader pari (Rasbora lateristriata Bleeker, 1854) organs. in Proceeding of the 4th International Conference on Biological Science 020007 (2016). doi:10.1063/1.4953481.

Gibson-Corley, K. N., Olivier, A. K. & Meyerholz, D. K. Principles for Valid Histopathologic Scoring in Research. Veterinary Pathology 50, 1007–1015 (2013).

Trefts, E., Gannon, M. & Wasserman, D. H. The liver. Current Biology 27, R1147–R1151 (2017).

Almazroo, O. A., Miah, M. K. & Venkataramanan, R. Drug Metabolism in the Liver. Clinics in Liver Disease 21, 1–20 (2017).

Hosack, T., Damry, D. & Biswas, S. Drug-induced liver injury: a comprehensive review. Therapeutic Advances in Gastroenterology 16, 175628482311634 (2023).

Hay, A. et al. Paracetamol and ibuprofen for the treatment of fever in children: the PITCH randomised controlled trial. Health Technology Assessment 13, (2009).

Freo, U., Ruocco, C., Valerio, A., Scagnol, I. & Nisoli, E. Paracetamol: A Review of Guideline Recommendations. Journal of Clinical Medicine 10, 3420 (2021).

Zusrina, L. M., Retnoaji, B. & Wijayanti, N. The Effect of Ethanolic Extract of Cashew Fruit Peel on The Liver Histological Structure in Rat (Rattus norvegicus Berkenhout, 1769). Journal of Tropical Biodiversity and Biotechnology 4, 113 (2019).

Hinson, J. A., Roberts, D. W. & James, L. P. Mechanisms of Acetaminophen-Induced Liver Necrosis. in Adverse Drug Reactions (ed. Uetrecht, J.) vol. 196 369–405 (Springer Berlin Heidelberg, 2010).

Davidson, D. G. & Eastham, W. N. Acute liver necrosis following overdose of paracetamol. British Medical Journal 2, 497–499 (1966).

Blazka, M. E., Elwell, M. R., Holladay, S. D., Wilson, R. E. & Luster, M. I. Histopathology of Acetaminophen-Induced Liver Changes: Role of Interleukin 1α and Tumor Necrosis Factor α. Toxicologic Pathology 24, 181–189 (1996).

Matus, G. N. et al. Behavior and histopathology as biomarkers for evaluation of the effects of paracetamol and propranolol in the neotropical fish species Phalloceros harpagos. Environmental Science and Pollution Research 25, 28601–28618 (2018).

Raja, D. S. S. & Bharathi, U. D. Histopathological and biochemical effects of paracetamol-induced toxicity in freshwater fish Labeo rohita. World Journal of Pharmaceutical Research 7, 1223–1233 (2018).

James, L., Sullivan, J. E. & Roberts, D. The proper use of acetaminophen. Paediatrics & Child Health 16, 544–547 (2011).

Downloads

Published

2023-06-30

How to Cite

1.
Septriani NI, Saribu RLCD, Apriliyani T, Karlina I, Pusparini NAO, Zusrina LM, Sari RVS, Allimi HS, Supraitno ME, Saeed F, Simanungkalit EE, Paramita P, Retnoaji B, Sofyantoro F, Wijayanti N. Histopathological evaluation of hepatic tissue of yellow Rasbora (Rasbora lateristriata) exposed to paracetamol. Biological. environ. pollut. [Internet]. 2023Jun.30 [cited 2024Jun.25];3(1):8-14. Available from: https://pubs.ascee.org/index.php/bioenvipo/article/view/595

Issue

Section

Articles