The effect of Garcinia mangostana L. skin decoct exposure on Zebrafish embryo Danio rerio (Hamilton, 1822)
DOI:
https://doi.org/10.31763/bioenvipo.v3i1.631Keywords:
Decoct, Embryo, Mangosteen skin, ZebrafishAbstract
Hypertension is one of the diseases with the highest prevalence in Indonesia and is the main cause of death in patients. Pregnant women have a risk of losing their baby due to hypertension or death. ACEI and ARB-type antihypertensive drugs are dangerous for pregnant women because they can cause fetal death. In ancient times, Indonesians have used plants as herbal medicines for generations. One of the plants that has medicinal properties is mangosteen skin (Garcinia mangostana L.) as an antihypertensive drug through its diuretic effects. However, the drugs consumed can have toxic effects if used in high concentrations. A teratogenic test is a toxic test that is specifically carried out to determine the presence of organ abnormalities in the fetus due to exposure to xenobiotic compounds. The zebrafish (Danio rerio) embryo was chosen as the research object because the chorion in the embryonic phase is transparent, so it is easy to observe. This study aimed to determine the effect of exposure to mangosteen rind decoction on zebrafish embryos' hatchability and spinal curvature. Observations were made using a Leica microscope with ANOVA data analysis. The observations showed that high treatment concentrations decreased heart rate, while high concentrations didn’t affect egg hatching rate, spine curvature, or pigment density.
References
Kristiyani, S. D. Laporan kasus: Hipertensi dalam kehamilan. Jurnal Medika Udayana 3 (2014).
Chobanian, A. V. et al. Seventh report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Hypertension 42, 1206-1252 (2003). https://doi.org:10.1161/01.HYP.0000107251.49515.c2
Gitawati, R. & Handayani, R. S. Profil konsumen obat tradisional terhadap ketanggapan akan adanya efek samping obat tradisional. Buletin Penelitian Sistem Kesehatan 11, 283-288 (2008).
Hasan, A. E. Z., Nashrianto, H. & Juhaeni, R. N. Optomasi kondisi untuk rendemen hasil ekstraksi kulit Manggis (Garcinia mangostana L.) Optimization of conditions for yield extraction of Mangosteen pericarp (Garcinia mangostana L.). FITOFARMAKA: Jurnal Ilmiah Farmasi 2, 153-159 (2012). https://doi.org:10.33751/jf.v2i2.170
Suksamrarn, S. et al. Antimycobacterial activity of prenylated xanthones from the fruits of Garcinia mangostana. Chem Pharm Bull (Tokyo) 51, 857-859 (2003). https://doi.org:10.1248/cpb.51.857
Nadila, F. Antihypertensive potential of chayote fruit extract for hypertension treatment. Majority: Medical Journal of Lampung University 3, 34-38 (2014).
Kumar, V. et al. α-Mangostin mediated pharmacological modulation of hepatic carbohydrate metabolism in diabetes induced wistar rat. Beni-Suef University Journal of Basic and Applied Sciences 5, 255-276 (2016). https://doi.org:10.1016/j.bjbas.2016.07.001
Choi, Y. H., Han, S. Y., Kim, Y. J., Kim, Y. M. & Chin, Y. W. Absorption, tissue distribution, tissue metabolism and safety of alpha-mangostin in mangosteen extract using mouse models. Food Chem Toxicol 66, 140-146 (2014). https://doi.org:10.1016/j.fct.2014.01.028
Suwignyo, A. Uji toksisitas ekstrak etanol kulit Manggis (Garcinia mangostana) pada embrio Zebrafish (Dario rerio) Sarjana thesis, Universitas Brawijaya, (2014).
Kittipaspallop, W., Taepavarapruk, P., Chanchao, C. & Pimtong, W. Acute toxicity and teratogenicity of alpha-mangostin in zebrafish embryos. Exp Biol Med (Maywood) 243, 1212-1219 (2018). https://doi.org:10.1177/1535370218819743
Athiroh, N. Dekokta scurulla atropurpurea terhadap kelengkungan tulang belakang embrio ikan Zebra. Biota 11, 13-25 (2018). https://doi.org:10.20414/jb.v11i1.82
Cockerham, L. G., Shane, B. S., Cockerham, L. G. & Shane, B. S. Basic environmental toxicology. (2019).
Nur, A. H. Uji toksisitas akut Decoct orthosiphon stamineus, Benth terhadap daya tetas dan malformasi organ embrio Danio rerio. Jurnal Kedokteran Komunitas 3 (2015).
Sinaga, R. N. & Siregar, N. S. in Accelerating the Achievement of Sustainable Development Goals for the Improvement and Equitable Distribution of Population Health (2016).
Sarasamma, S. et al. Zinc chloride exposure inhibits brain acetylcholine levels, produces neurotoxic signatures, and diminishes memory and motor activities in adult Zebrafish. Int J Mol Sci 19 (2018). https://doi.org:10.3390/ijms19103195
Piacham, T., Isarankura-Na-Ayudhya, C. & Prachayasittikul, V. Quercetin-imprinted polymer for anthocyanin extraction from mangosteen pericarp. Mater Sci Eng C Mater Biol Appl 51, 127-131 (2015). https://doi.org:10.1016/j.msec.2015.02.051
Kowan, K. A., Airlangga, H. & Aini, N. Uji nilai LC50 dekokta Centella asiatica terhadap frekuensi denyut jantung embrio ikan Zebra (Danio rerio). Jurnal Kedokteran Komunitas 3, 147-155 (2015).
Sanchez, M. et al. Quercetin downregulates NADPH oxidase, increases eNOS activity and prevents endothelial dysfunction in spontaneously hypertensive rats. J Hypertens 24, 75-84 (2006). https://doi.org:10.1097/01.hjh.0000198029.22472.d9
Landmesser, U. et al. Role of p47(phox) in vascular oxidative stress and hypertension caused by angiotensin II. Hypertension 40, 511-515 (2002). https://doi.org:10.1161/01.hyp.0000032100.23772.98
Yumnamcha, T., Roy, D., Devi, M. D. & Nongthomba, U. Evaluation of developmental toxicity and apoptotic induction of the aqueous extract of Millettia pachycarpausing zebrafish as model organism. Toxicological & Environmental Chemistry 97, 1363-1381 (2015). https://doi.org:10.1080/02772248.2015.1093750
Sant, K. E. & Timme-Laragy, A. R. Zebrafish as a model for toxicological perturbation of yolk and nutrition in the early embryo. Curr Environ Health Rep 5, 125-133 (2018). https://doi.org:10.1007/s40572-018-0183-2
Park, J. et al. Acoustic radiation force impulse (ARFI) imaging of zebrafish embryo by high-frequency coded excitation sequence. Ann Biomed Eng 40, 907-915 (2012). https://doi.org:10.1007/s10439-011-0466-3
Rahmawati, R. & Kadarini, T. Pengaruh warna wadah pada pertumbuhan dan perkembangan larva ikan rainbow kurumoi (Melanotaenia parva). Jurnal Riset Akuakultur 13 (2018). https://doi.org:10.15578/jra.13.2.2018.137-146
Clotfelter, E. D., Ardia, D. R. & McGraw, K. J. Red fish, blue fish: trade-offs between pigmentation and immunity in Betta splendens. Behavioral Ecology 18, 1139-1145 (2007). https://doi.org:10.1093/beheco/arm090
Dewi, I. D. A. D. Y., Astuti, K. W. & Warditiani, N. K. Identifikasi kandungan kimia ekstrak kulit buah Manggis (Garcinia mangostana L.). Jurnal Farmasi Udayana 2, 13-18 (2013).
Rita, W. S., Suirta, I. W. & Sabikin, A. Isolasi dan identifikasi senyawa yang berpotensi sebagai antitumor pada daging buah pare (Momordica charantia L.). Jurnal Kimia 2, 1-6 (2008).
Staal, Y. C. M. et al. Head skeleton malformations in zebrafish (Danio rerio) to assess adverse effects of mixtures of compounds. Arch Toxicol 92, 3549-3564 (2018). https://doi.org:10.1007/s00204-018-2320-y
Wu, T. et al. Icaritin, a novel plant-derived osteoinductive agent, enhances the osteogenic differentiation of human bone marrow- and human adipose tissue-derived mesenchymal stem cells. Int J Mol Med 39, 984-992 (2017). https://doi.org:10.3892/ijmm.2017.2906
Wiendarlina, I. Y., Herlina, N. & Mareta, E. Toksisitas akut sediaan cair berbasis bawang putih dengan metode Zebrafish embryo toxicity (Zfet). FITOFARMAKA: Jurnal Ilmiah Farmasi 12, 78-88 (2022). https://doi.org:10.33751/jf.v12i1.3883
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